	   SnapGene      nCCTTAATTAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCGGAATTCGCCCTTAAGCTAGGATGCTAATACGACTCACTATAGGGCCATCAGTCGCCGGTCCCAAGCCCGGATAAAATGGGAGGGGGCGGGAAACCGCCTAACCATGCCGACTGATGGCAGAGCGGCCGCGTCGACGGGCCCGCGGAATTCCGCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGGATCCAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGCCATCAGTCGGCGTGGACTGTAGAACACTGCCAATGCCGGTCCCAAGCCCGGATAAAAGTGGAGGGTACAGTCCACGCTTTTTTTCTCGAGTTAAGGGCGAATTCCCGATAAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTTAATTAACCTAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAGATTTAATTAAGG  0Ý                                                 $            (                                                                              
                                   %               "      &                        
      	         *                           Å                                             
      	                                                                                       "                                  !                                 	               	                  
                   /               %                                                            %                                    -                        "                           %                              +   !                                     !          &      "      #   ,         „ <�ÐO|ä¼½ù78Q~µ¶·[\¬­® m  0‚^œG¢
 �ú9TP] … Ñ„	Þ	BÊüÞê7€¸8| * 5 < ò PÙ=½.P}åò¤	/
I
T
¾iR¬¯Ë n ÃÎ=[K5�	
8[ß(€ÐCQ³ À È !X•ß7Fxå¿ò	£
`ûeU=^¼kqÄ&D Û Û1_‚“Ó<w	µ	Žù¼:Ûå�
lÂš2 ƒêã@¸o�ßHaS	!Nò…!  �D´ÒÕšVCHýìÀß.¯³ !¯…	S@[Åà&-h † b‰"xß	KË#¦ë˜¶�¹}Ú¸ G #áBk	:
wx8¨9  6 = †»¾î{éQ¼N	¥	Û	0
U
­
Þ
1?N°âk ° + <Ïr¾*Kê/DQ’¼jI~óàï™jÀ”É‡4¯Ìø 	 óÃæë$|Æ	J
£¿ç^­$ " O s ó DQ‡œÚç˜>r¨ú^ï=q„
+
X
ˆ
î
žøSÏáìš   k ×`Áÿ>¶3‚	)
ì
àNi i 1<Í©vèÿD o Öê\L6�	
9ioæœé�?0]æ( X \ ` @ø·ÄåÏ&!sÀ	a
e
i
±5o\w)1OÉñÑøFR‘´4TeuÒ. Á ‹48GQ8žÀ+‹3–é
fÓ!eÝlr "àæ”£¤
ü_¸Ã®˜`_½ÅE É Y–+¨	Ë�Ïa' yzŽ�ÜÀ[	ó	aô$V³”+±Õã>L Ü 9�þ	 Í2½…ÜX‘¡ ­Ü Ôx	°	Ï	Jíæ«!øn�›ê93 — ”§=Ñ÷„�˜¶ºêî˜žV` Ä 27¦:`ƒ¶	ø
Öú;M¢emxÃ Mõ„ëñäçA¹ìpiIB
" 2ct{� T‰ƒàù>Å}ž"´ ç 5¨5«žT	ž
O“×†ŒÀ @ g2kt–õÎ(Tb3
ZèóÐ,WÙPP q ã‡ÓÑ;OTQ		FILíèL5´\i$ V Õ2Ôµi›R¡ü	_
œ
XþÁë{/=öŸ2cÐä, ¡¥V	W`0D€v" £ŽÊEÖW~ƒÂõY×àÙ°¾ Ž ² bX	Ì	Ô	,
Œm]2 ô~™á
ÇÉA\ÆŠá. §,°êþ0kqT^Ah'i" "^x†	ž (ÑØà¾#8Byï2
	5Û™Q�ì‚— ‘ �³cŸ	à	°
L§ìpU”è §XŠb�
$©(ÖA·»~¹ ‡ >É;NœÌAVBGºÛü 9 H û J/]â
K	;
Œ
™
‚ys©:y•
 ìEŽ­¨ƒÌS¨_ «Èí$^mt)�x9x ³ÉCLl	‹	;t  BVv–¼½E_o‚Ü	V
/=@—Qöã>±¦2±ÍoÉ0 > Z ^ e‡¿ïê8¦	1
c
g
ç
É±lÓ[g 7 H¥ÿ|ÀRŽO	®
ß
Vy3Qˆô“•    b d Ñú6C

k
m
%2;QksO ý†ó¡HVw“Ô f =;os€¡ˆ½ÄÙo
Å
SÖïIîõ£5FX©Í , Ð¶¹+LëE#Jlkq�•¢Êˆ˜È‚ÖÛ ú!0“kÂ	×	4š³ÁÀ ¿GC¤ÓR[ôÈ
ó
áðâ7.L5ÀJ°ù% 
 Æç/P(X÷úHuÙ&fÙÜì%vÛ
³½ÐÛ^¦‘_b®é6¬ ¥ × ß ŽÄÌñTÇ	^GªËLúK ‘JM…Ü÷¥‘	K
‚¤ÙàQ&Â”èÌT    º ôÇÁ’ç(}

"
y²À6¯ÿG¤ô?ì # þ 2EˆŽ�Å‹ü€ËX[Éyh	,
‰
 •f|+¿CI›" t   µ ô ™ Ì©û!·l«„·5D	b	
×
g—ÞùhTeƒÄ\ P (�?äÌÐ,r…Y
r
À
Ÿ¡Hˆ²:d¶Ðâí*‰& & 1 K Ï 	R`ÛèsØSY_¼ðý>Õs	>
E
P
ï
ü
'mu+…`Çy l ƒ ñ Ø¼-ãƒ	£	ÉûŒ7OZ« ¿ Ú 6ÂÍâ<?·�4´	ñ	dñ	kBjC "Œ a ™9
ë®ß%·  ! ›qºqÂH*
í
á†Ÿ÷  h j ?é¿	ë
hnÌÈðEt Š�Û2Z	·#Ž­Î_*  Ã ¬¥¦Î	ÌÕ
W¡ªédw÷é_
 Sô'=Ö�ÏVŒ U p É‚~	û	^
ÖKçØê[h1bÏ	 ]}˜Ó	jpÆ�È � =×ß:M7ž	
ÚAëoÕ@“ç ú ë\²J	Š	:§‚rs^* Y ] a AduþÐ¿5^b
f
j
æ
.:Ujrx2P°ò¥Òó1G’UZfnvÈÓ/ ù:¸ £‡Ø"Á	Ö	Ç
6p²ŽK¿í4Ç Ö IÅæ.O'WÆt‘Ú
±²]ÁÊËùS5«ëJ 0 ´ �ŸŠkr	q
g*_ÆµH š!5¹
»Áá,;˜€¢	³	ð	8
‹cjÞži Â R«èóŒ¤&¥ê
gËÔ	"mËcÇ)‹s  9±|—}	Ò	Å@êÔq / Õ Œ9Hžý-NŸV†4q	9°±f^¾0mÇ . �¸ç%¤|	7
bÄÊ?°ébiÆÅ œfgh•–š›œ�žŸ ¡¢£¤¥¦§¨©ª«¬­®¯°±²³´µ¶·¸¹º»¼½¾¿ÀÁÂÃÄÅÆÇÈÉÊËÌÍÎÏÐÑÒÓÔÕÖ×ØÙÚÛÜÝÞßàáâãäåæçèéêëìíîïðñòóôõö÷øùúûüýþÿ 											
																						 	!	"	#	$	%	&	'	(	)	*	+	,	-	.	/	0	1	2	3	4	5	6	7	8	9	:	;	<	=	>	{	¥
îïðª«¬¾¿Õ`aÉ¯ó0ažŸ ¡¾¿ ï›·­ÀÖý *M"™1þ #áWi—ö?	¦
ñ[¹p¤ôš¢`À„F ” Ò,ðáe	®þN/�b‘¦á â —£ã
H×!+AVYÓšÿ}  Ê Zœü©	w
€
ÁÌæÐÏÝ(9e ˆYã	¼
¸#$2ŒO ~[j÷q�.L–
ÆÝº%
qš•:²ÖaÁÊMØ X˜Gh°Ý\	be»Òw ð2—Ì›?6G w íbž¸Á@	ê	·
ò\õsüC¥¬´ŠÈõÑã,ä › $,âzÃ{®Œnô	{
§
l	Dã"W£… :ñ.¦²}@m Ä	Ù	ÿ	¾5’ÝYŽº • žœ1¯:m03‘Ãçc’*ýNT ±Ó-T¼Ïÿ.J?úâ £ ¸ Ý 0Kºâïf	OÎÔ†¢§
 ï Ýä
IÔ›¬‘q ˜©¡y	±	Ð	‰BKWáîç "oœ4	 ¯À¤xØ,ZÞà ã ®Õ"�kâù @ë:~ Â)¨ô�´£\`FÂë)  ˜ •ç	x
6øÚVÑ©Ð»$£ÞôWf ¶�	Ò…�™·ÂÍçzœïû™:a Ë [ü²ý>ª	�
|&s‹ˆŸ Å #2³æÆ½
ù
¹ñNj£ } ÷ z•;a‰ìG	ä	×<CfÄ ¬ 38?Ø·	rûµ$s%n�k ÜJ§„ZÌ
2Y3yP) Nìø(yèB�ºíºÞ!º|³Ø å ö›¦å/qäruƒy–Ž×LN² ° \£Ïk…òÇrMJÊ	—
š3?&›; Ê×j�	C
#OÇÞ»d
bÂË€ÙZ Šiáˆ	«ÆŸÄñ#@µAH UYÁ¬þñT`fo„®z˜úWî	”
c¼Óä?0˜Íœ7 IRšH±>Ê
$xï3>ø °™Þ]	afƒñ~wÂÞ?  6„¬]uöA	U	_	&
…
Ÿ
]öó­ØN·ÉLäåÁ € ì îcÒ¹ë	…¦µ‹ ¨ ©6ŸŸ¸
cóò9”ý¸÷äö$�	 x è dÂ
Pht D¼ÚÒ-‡ð %ÍHUõ
[#X§7}—à'<Q† -½|¯El—ÕÏÕ‡4
¤äm€ôÑ-¤­Îû!XQ A ã‚²$u�Š8c|
ãBÚí† œ h{Ä$3co‘öà)oõ	¨
³
ém
E³Xû /;N{ñ¡
Ýé6S6P ”EÚäéZJn’ÒÞ;R	€	 
7M_øÚÞ'�„Ï»Ã% F ˆ³ÔàA®!UwGJ¥¿—ZÄµg" r ;†Îæ…ò§]~<P#Å	Ú	
‡
¬
Ý
îM¿“¯æ]jàë™®   W ÖÕ¶ 2(
`
°;n4›è0¨>Nuç÷þ�/3dÑå- *3P“7�*œ¢^ÂÒ`d“° – 86Ÿý	öÿƒµI1ìL¹í  8U ô1jsSªhø�
Y1b’Ä|+‹O ¢ÔÓâU Ð:X_/E Kžã!#+ � a¦+wéW	+/@‹1 ²'WÈ½aî1U¨ºTû ªì.
(D�	�Ëwx  ¤•B�
–õŸü… nµFBÃZÄ
áÕ-îH—¿¨! ¤ ¹ Þ L„öùGÀÛðö%eÛ'SuØP®ÏFÚ�“Ka£èó 1Ä�Ë× RX»üãƒ¶ÏÔ4xC	a	g	O
f” Ý‡±¾d  ‚ ¾ Ù ð pÈúê­ö Y	¾	Í	Õœè^ � Þ[ú	ÕJn’r ³ cÕ	å
-q�À3ê Ô ™ª¡	²	Ñ	8ŠÊ]½p�/h eõšz	¶©LÈ>è!ò  “ á ¢àâ	v

»
â
GXÒ #‹N¥àd š +}·é	z
ºÅÑÜCâï!B«Ç9â5 °-¥»Îÿ0-lIÓ�ü¹M	 î ®¨wˆßáp µÁ±ó³{¢Ù[rŠU_‡¨# « yëq1XBir(j
 ¯ ä 'xÆÉÖÉ	NÝ¹×Y ¯Qð_ey—V‡	í	“
`ã#î‚v=Ýð	 § ƒŸ ·»Ùã #�Ô
²
l¬ÍúAW|–ì&; E “ÍÙzð"
6¤Ü–RÃƒÎ˜ ó)O’©õ0‚]ašKìt�. Œ ©Òá*Ÿè9CT¹� * Ãm¿õ$d‚µÓ3N
Ã

e“•Ÿ­,žíG–½çò
  ZÇÔùéçéõ   ’ ™ Ó d¶ß 	á	è	u
y
º
7F¨¹ÄÐÛ=ªÑ¼ñÿ8¤ßcg ª ´/‡‰WÒqo	 ‚P–È’
±
MÜâí¸ÿºØV{•Ö%X D ¨Ìl‘žçcM
/�’”ž¬+¸¼œæñ- OYÇÞ.‘
ÆÓÛE†¸ÃÏè<×æðþnÕb/ ·Ë‹Æ
“	”	•	–	�
%&€‘Îô‘’“�ª«);()*üýB}~€Ö×š›ÓÔåð ¸	Œ—¡§—	Ó
~„‘¦'šõ{*C�Ø º; Ì O¢“Ô
�(›öD»€ Ê<‡®Þ8¡‚
'Støê& < ðL«Û³?P˜	…’B}C‰ùq‚	ý% ˆ \¹ý
?�ù˜¨þfjŠ«	º	 ˆ�§ÍW|Œ¤+ÄHÙVÜ» : Æ BçhéL	ºuœ÷vª;z K^ã	$3´”›	æò– 0gP�
š
OkEt¼¦   I Í ü É£Ç*
 
<
¾
Õ
ú
€•ž)ƒzF<¤E�A S ø ˆF�H	\
ØŒ„ÍTë©` íû¯ß9–¢Š®j(TuþgÅ'‰ ={íÄå	8=DÜù(¡ò  ~ ê Ë<b$
ƒ
±¢©µöî= %C_“49@�@Q
Dú%ƒŽ/I‰ ­ ¼ ¬nu´y¾t
 ÉîñM¬ÙÜ´,ˆ‘¸	†CR~¶orÔylþ löf*™	
“süŠ:&Œ ]´¨™ÿ‹Î3}	Ú* z ºÊ…[Œ	<X,IW ‰ þ@øŽÝKDÃ”Mg»	{Ž¥zQuÝ nrú©¯km	¬	
Í
‰¨Â�ZÅd4Ž¼ ) 4 ; O€Ï]›FÝ	.
H
S
A»øh}«´{Ê Ç WÞw¾`v	»ZäÁ™ <²p1  ŸCj	"vRw-?²§,Ù% N C—½íù)JzéC‘»èîi»êp{ƒM	W
0>¢ß�˜÷È3Î [ _ ÷_Àäþ
%µ0rœ	d
h
åvMS\h Šy¿•§	è
Êó ²—ÜÔâK œ9¯	÷
Ð—„m�—� ? æ 4Lfˆð•§æ4‚Íë 2
A
çszØO³¿ 1¤†Vh}ÁôQNSP	›
Wz4<u½õ ±  óýË	à
	]gl\‰! €¦ÐAŽ
¯
›4@'PœF– 8 IÇ]lÈsK�‹
˜
¬R”§  c GUù7Dn�
l
$<PÈP=‡Ì’” e Ò kn
ò
3R~¡ô¢IEW�ÕÚ    ��ËØ¤Ø�	

!
]x�ß5¼%þ¥©F>- % J Ÿ Î ý '_¶ûªÊWZÈË+=
D
¿
Ö
û
&lt–Ÿ*„e{G9c‚Ã)Bxˆ[ p‹ÌGâð…$6AYª¶ÿB g >‰Ú¶�¬V ÎUïö^ T ù <È�I	‰	]
Ù¦Ç×ZgaÎ t‰¢j¾Å×�]p
Æ
Ù
TiJ¯ì¤ÅÊòÆGYªI  ü	ºÿ,M°àò:U…—£{p	ï	¯lÓÆ(Š - V›·î$™6
íZ½Ô)o£/h™�ýƒ Ñ‡ûXd	å@U.�Î|Ü8" v ZÂ­ìFagp¯-‹Km•
¶
dkvÿrû	1–™Ë‰ÐÉ× ¢ · /JS±›¹|?lîÃ	Ø	9Âæ4>)ùS ¿€àjÝ? û(€æ	5%y›EÁú¢ó | ö Û>×"1I”²åÅF	Ë
ð´ ¢Î~š¥¹Ž	™>ã‚x	K± ÀH«ý™Sn­=É
ª/ÁÃ÷>@   ë Ñc\tõ^	

%
„
bgñ8„òöM¶K#ï ¼�±Ì#DGbnÔß†‰7ô
â²£¦ 6ß…ú% …åD„:ßè\MYI‘­ vÝ:†
 
«
Ü
^¾÷®Ù&Oåfßê­Â  7506r)g÷'
´HÑ_§¸M¯æŠ7 ë&`Çv'^Œí.V	*§ŠÊ0Sw  ”´AQøûFÚÎÚ&ARtw€B	`	
EÜôû„�’J`c � ½ Ø  o½	ù	p¿ à *|~
µ M í ­vºÍò²pz¡H†§Œû¸L ¦ ® &w�ÅïdÓ	UÈ	ì	_¶u«Ì<Üï ‹ ’òNÒ!Â
dôS•Js•ÍŽ  © Ò µt
¹
áˆ·þ¹7z+ C ÊkN�ÅÝæ-– ¦’	L
Ò
}ƒ�¥ÅÚ.Dó�:'å|ÕmÒäïÿ,
! ïK†ª­ÚÝ7 øOei‰’¹	ÿ
‡ŒR£Ãø÷pé%U   AfÈgè)š	

”åtuE ;D¥@  R é úÃ#
[
i7°ý‹¡´Û'ˆõíñ » kB’õe�+~‡

³Q½Ó.Hˆ‹ y mq÷Â“®
zÁc� ( 3 M ÿ žÝÎI\šzi	u	-
G
R
¡gYÇ
,À~˜+ 3‰x”¾ý�Ìÿ®	@
ö
Ï–ÛáJRœ¾ ÆUóü@ Š
«~\f[   $ ž &FT^�Œµø©VYÊ×mŒñ
\w4}$¨8(=D‡‘ ÇËÖ\Ø
hµ¥U®YfÉXÅþ] u š,†c	o	5
µ
ìY-únÒ.Ïü{ { ¡ ¶ õ .¾'°Ú¸ÖäE	ßåiR! »€¡Í}ªü"Fam˜m¬Ðs…ˆ¸6�		
˜=�õJ¥"„  äêƒ³Þ5[qLE‹Í-9sª
)G¦‰RevÞ ){ÿ%v±¼	ø	}
oo ;Ûî·&   B Š Ñ j�Äå•Ñ Ndhs
Á
Ñ
þ
|‹�Ä-‡�¢I6{Ñãî+ Q jpA‘ù@eŽ­†
Z
äb³C‡Š" ' 2 L � 
SÜwT“½÷òþû?Ölt	­	?
F
Q
ð
vª[ÆeZ�½ Æ&Ù`ã+rn	´
ë´Èzý# Ð iozéi4Zdp¬°÷	
©
Ð
ý
(nŠÃ,nF†Ža5, abcdefghstuvwxy|}~Å%tuv’áâñ*pqª«ö	Î
Ï
©êY�ü  ,Â  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                        %                
                                                                                           
                                                       	                                                      -   !                                 
   
   	                  
                        	   
                                                            %         c   6         P   Q                L                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
                                                                      %      
                                   
                     	                  - !           
 
 	      
        	 
                      %   c 6   P Q      L   d?  e5  eM  es  hÍ  lï  s/2Œ'sÎ  3 { 3Æi}'xUøV@sÖhsE	n	ø	@
(n+Ñã‘@ 3
{Ìb8Z¿8$q å# �P
à°	°	†8  # ˆ‡�¶CP|…RÌÐ,y…P
ó
24^y—ˆÄ6yÍùªå˜@ à3
{@ 3
1{@ 3
{2«Ìb”yàÚôÁÿ9?†Bä[Ã¢u
cI& 1 E
P
±rØ<qÄ 24Á Ó¶	mÊ™Ê‡jIJŸ²!��!Õà¯øw4=@.~%šr C†—§ù~
‡
÷™É Y›¨	ÀË6)Ñ†é¢6 IB
cÒ £Rø„EYà5¾EYà¾' 2 š	F
Q
PÙÐ£Þÿ“PÙEÖWPYòà¾r §
‚PÙå¾ãKfQí�0'ÓŸ
NÆí `V	*Š0ïjrv† PÙx�!<rÕï‚k	ò	¿ËàU(Q¯³ÚøYI&sŽúˆ÷
8¯ ¢É	' 2 Tÿ@}°ÎZýL¸  mF
Q
©
XýúX—*Ð�€Eþ �Hyh	 + b 
k
Á3÷CÈ~ÂAC÷
w§O°PÙEÖWPYåò¾à5¾w8(Røÿ° ‡é
fr   Z ^ b Ro+L|ÀÇÌÕ(ry


c
g
k
×
Û
ï
gu ”Äé?¬ÉT}°¸ mIRø¯ É	1¥‚µ	ù

lwÅ€ÖÍ^	ò' 2 F
Q
Tm<ð¼â°]´¨6)YIÑ&†é×ìøFÚt�`É Y¨	JHJEÉô›YQ	‹	;LàÖ¾ñ0CMg¢P�™	¬	š
8%(rÅ'‰¼Ø5‚:y Tt×]mÙ
¯¤ªügè š	Ù
ªJÒ úš	sˆŽ×YPuv]w�³ +âÅ	J¥1_‚µ	ù:
lÂ¦ƒx)O’®Þ8¡'S]t&äƒÞ[ËL9ª
XeÞþ)9<
 sP¡½Ä÷úÙÙ%vo
Å
SÛI‘_b£FX©ò PQÙÚç=r^ïB=ak	î
òR' ( 2 3 L 
ÜÎ\št	-
F
G
Q
R
gÆ~´šýÀ.Ë±¢Bak	òs¡½Äo
Å
SI£FX©
 P÷úÙÙ%vÛ‘_b{ õ ÚÖäE	9þ	 ½ÜXìø(yèB�ºíºÞÅ‰

 # BEŽs–¼P‹¡½Ä÷úXÙÉÙ%ov‚V
o
Å
/=S—|ÛöI‘_b£2ÍCFX©' ( 2 3 L 
ÜäƒÎÞ[ËL\š9t	-
F
G
Q
R
ª
gÆ~XeÞþ!0“³Ü Í…¡„¬u…
Ÿ
]ö­ØNäÁÇ¥YfÛ ¼Û�ÛIË
R [
‹ '
§Mæ‚œG l	]U	ˆ	!ÆÉØ R '
[
‹§MæT È�]
×ZgaÎ \tõ^	%
„
òM¶KiB
"c7 HŽQ“� S÷Ölð
v�EÜ	@Éç ê=9O�†]ž8 ILðæëA
‹
˜
sz”Æ 4ÎŒÍTÚ;‰Ndhþ
‹¢4À+ÝÁ’yÀÃÎ[(ÐQ³�iRœ
°
LXì¨A{ÆáU”Á Q‹é
fÓ!rî¬ÙÜˆ‘¸	†oê9�]ç O†iRœ
X¨{=ž# BEŽ–¼‹XÉo‚V
/=—|ö2ÍC T È�\tõ^	%
]
„
×òM¶KZgaÎœc’<ø ºÿ} c GUù7Dn�
l
$<PÈP=‡Ì’”$ ž &FT^�Œµø©VYÊ×mŒñ
\w4}$¨8(=D‡‘BVv–¼½E_o‚Ü	V
/=@—Qöã>±¦2±ÍoÉ0"^x†	žÖ IÅæ.O'WÆt‘Ú
±²]ÁÊËùS5«ëJ  b d Ñú6C

k
m
%2;QksO ý†ó¡HVw“Ô� ½ Ø  o½	ù	p¿Ä 27¦:`ƒ¶	ø
Öú;M¢emxÃ" O s ó DQ‡œÚç˜>r¨ú^ï=q„
+
X
ˆ
î
žøSÏáìšk( 3 M ÿ žÝÎI\šzi	u	-
G
R
¡gYÇ
,À~˜+¢ · /JS±›¹|?lîÃ	Ø	9Âæ4>)ùS	 " 0 O s ´ Ö ó DQ‡�œÚçI˜Ÿó>r¨Åæú.O^ŠÃ'WkÆït=‘æë$q|„r	Æ	
+
J
X
q
ˆ
Ú
î
£±¿ž²ø]gÁ*S_Êç^­ÆËùSµÏáì5Hš«ëJk¢ · î /®°¿¨-JS¥±›¹w|?l»Îîÿ0Ã	Ø	ˆ-9lI€ÂÓàæ4>j�Ýßá)?üp¹ùMS° \£Ïk…òÇrMJÊ	—
š3?&›;nµFBÃZÄ
áÕ-îH—¿¨( 3 M ÿ žÝäêƒ³ÇÎÞ5I[qËLÖE\‹šÍ-9\szi	u	-
G
R
ª
Ø
)h¡G¦µg¥Y‰Ç
,U®ÀRYf~˜É+XevÅÞþ]( 3 M ÿ …žÝåDp„‹:ÎßèI\ÌMYI\‘­ GvšÝâ:zi	u	-
G
R
†
 
«
Ü
¡^¾÷gY®ÇÙð
&,O…Àå~˜$+6AYfªßê­¶ÂÿB Ê Zœü©	w
€
ÁÌæÐÏÝ(9eQ jpA‘ù@eŽ­†
Z
äb³C‡Š  k ×`Áÿ>¶3‚	)
ì
àNi ’ ™ Ó d¶ß 	á	è	u
y
º
7F¨¹ÄÐÛ=ªÑ¼ñÿ8¤ßcgA ° Ö ã"I\‚Œ£²Ï ak…Åæò$.OuÇ 'Wr™Æt�MŠ‘8JcÊ	9
|
—
Ú
š±ã3?²ë&]›Á®;ÊßBËÚíù%S†5«·ëJK^ã	$3´”›	æò–   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5443-5459" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5419-5435" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2694-2710" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="226-244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5345-5366" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="5381-5386" color="#ffffff" type="standard"/><Segment range="5387-5404" color="#ffffff" type="standard"/><Segment name="-10" range="5405-5411" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3333-3437" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2852-3307" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="IRES2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="361-934" color="#a6acb3" type="standard"/><Segment name="ATG" range="935-937" color="#a6acb3" type="standard"/><Segment range="938-946" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4469-5057" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="947-949" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="950-952" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="953-1666" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3506" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="3438-3506" color="#ccffcc" type="standard" translated="1"/><Segment range="3507-4298" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
   <Notes>
<UUID>74275b94-e19f-41d2-b834-eef876e49733</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>Circular-ic-EMCV-GFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="8:9:14">2025.8.11</Created>
<LastModified UTC="12:57:19">2026.3.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      HO ÿþ               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
